Enzyme-linked Immunosorbent Assay:
Article Title: DNA methylation-mediated silencing of MEOX1 promotes glycolysis and immune evasion in colorectal cancer cells through inhibition of GLP2R transcription.
Article Snippet: .. Supernatants of immune cells were collected after CM treatment and assayed by IFN-γ ELISA Kit (E-EL-H0108, Elabscience Biotechnology Co., Ltd., Wuhan, Hubei, China), TNF-α ELISA Kit (E-EL-H0109, Elabscience), and IL-12 p70 ELISA Kit (abs510012, Absin, Shanghai, China) for the release of pro-inflammatory cytokines. .. Orthotopic tumor tissues harvested from mice were analyzed using the mouse IFN-γ ELISA Kit (E-EL-M0048, Elabscience), mouse TNF-α ELISA Kit (E-EL-M3063, Elabscience), mouse GZMB ELISA Kit (E-EL-M0594, Elabscience), and mouse IL-12p70 ELISA Kit (abs552806, Absin).
Article Title: Sodium thiosulfate attenuates liver injury in a rat model of sepsis by modulating ferroptosis and oxidative stress
Article Snippet: .. Plasma levels of interleukin-6 (IL-6) (detection range: 12.5–800 pg/mL; sensitivity: 7.5 pg/mL; catalog number: E-EL_R0015) and tumor necrosis factor-alpha (TNF-α) (detection range: 15.63–1000 pg/mL; sensitivity: 9.38 pg/mL; catalog number: E-EL-R2856) were measured using commercially available ELISA kits (ElabScience, Houston, Texas, USA) following the manufacturer’s instructions. .. Absorbance readings were obtained with an 800 TS ELISA microplate reader (Agilent Technologies Inc., Santa Clara, CA, USA), and plate washing was performed using a Biotek Microplate 50 TS washer (Agilent Technologies Inc., Santa Clara, CA, USA).
Article Title: Sodium thiosulfate attenuates liver injury in a rat model of sepsis by modulating ferroptosis and oxidative stress.
Article Snippet: .. Plasma levels of interleukin-6 (IL-6) (detection range: 12.5–800 pg/mL; sensitivity: 7.5 pg/mL; catalog number: EEL_R0015) and tumor necrosis factor-alpha (TNF-α) (detection range: 15.63–1000 pg/mL; sensitivity: 9.38 pg/mL; catalog number: E-EL-R2856) were measured using commercially available ELISA kits (ElabScience, Houston, Texas, USA) following the manufacturer’s instructions. .. Absorbance readings were obtained with an 800 TS ELISA microplate reader (Agilent Technologies Inc., Santa Clara, CA, USA), and plate washing was performed using a Biotek Microplate 50 TS washer (Agilent Technologies Inc., Santa Clara, CA, USA).
Article Title: Programmable nanomicelles rewire myeloid immunity for durable control of primary and metastatic breast cancer.
Article Snippet: .. Enzyme linked immunosorbent assay (ELISA) kits were applied to evaluate the cytokine levels of murine TGF-β (KE10005, Proteintech), murine IL-10 (E-EL-M0046c, Elabscience), murine TNF (E-EL-M3063, Elabscience), murine IL-12 (KE10014, Proteintech), murine HMGB1 (E-EL-M0676, Elabscience), human TGF-β (E-EL-0162, Elabscience), human IL-10 (E-EL-H6154, Elabscience), human IL-12 (E-ELH0150, Elabscience), human TNF (E-EL-H0109, Elabscience), human IFN-γ (E-EL-H0108, Elabscience) and human granzyme B (E-EL-H1617, Elabscience), in accordance with the manufacturer’s instructions. ..
Clinical Proteomics:Article Title: Sodium thiosulfate attenuates liver injury in a rat model of sepsis by modulating ferroptosis and oxidative stress
Article Snippet: .. Plasma levels of interleukin-6 (IL-6) (detection range: 12.5–800 pg/mL; sensitivity: 7.5 pg/mL; catalog number: E-EL_R0015) and tumor necrosis factor-alpha (TNF-α) (detection range: 15.63–1000 pg/mL; sensitivity: 9.38 pg/mL; catalog number: E-EL-R2856) were measured using commercially available ELISA kits (ElabScience, Houston, Texas, USA) following the manufacturer’s instructions. .. Absorbance readings were obtained with an 800 TS ELISA microplate reader (Agilent Technologies Inc., Santa Clara, CA, USA), and plate washing was performed using a Biotek Microplate 50 TS washer (Agilent Technologies Inc., Santa Clara, CA, USA).
Article Title: Sodium thiosulfate attenuates liver injury in a rat model of sepsis by modulating ferroptosis and oxidative stress.
Article Snippet: .. Plasma levels of interleukin-6 (IL-6) (detection range: 12.5–800 pg/mL; sensitivity: 7.5 pg/mL; catalog number: EEL_R0015) and tumor necrosis factor-alpha (TNF-α) (detection range: 15.63–1000 pg/mL; sensitivity: 9.38 pg/mL; catalog number: E-EL-R2856) were measured using commercially available ELISA kits (ElabScience, Houston, Texas, USA) following the manufacturer’s instructions. .. Absorbance readings were obtained with an 800 TS ELISA microplate reader (Agilent Technologies Inc., Santa Clara, CA, USA), and plate washing was performed using a Biotek Microplate 50 TS washer (Agilent Technologies Inc., Santa Clara, CA, USA).
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